Interfacial behaviour of bovine testis hyaluronidase.

Crystal structure determination of hyaluronidase, a major bee venom allergen, in complex with an IgG Fab fragment and purification and biophysical characterization of bovine testes hyaluronidase

Occurrence of hyaluronidase inhibitors in fractions of electrophoretically separated serum.

An inhibitor to the action of hyaluronidase obtained from bovine testis has recently been described in human sera, and has been found to be associated with electrophoretically separated albumin (1). Another inhibitor of hyaluronidase has been described recently which inhibits streptococcal hyaluronidase and which gives evidence of being a true neutralizing antibody (24). Because of differences ...

The soluble hyaluronidase from bull testes is a fragment of the membrane-bound PH-20 enzyme.

The membrane-bound PH-20 hyaluronidase is known to be essential for fertilization. Here we addressed the question whether the soluble hyaluronidase from bull teste is related to the PH-20 polypeptide. The sequence of the membrane-bound PH-20 hyaluronidase from bovine sperm was determined via cDNA cloning. In parallel, from a commercial preparation of bovine hyaluronidase the major 60-kDa form w...

Investigation of aminomethyl indole derivatives as hyaluronidase inhibitors.

Hyaluronidase inhibitors are of potential therapeutic value for the treatment of a variety of diseases, such as cancer, arthrosis, or bacterial infections. Potent and selective hyaluronidase inhibitors are not known so far, and current approaches to the development of hyaluronidase inhibitors are limited. Elevated levels of hyaluronan (HA) are connected with most malignant tumours. Therefore, t...

Determination of the chondroitinase activity of bovine testicular preparations.

A simple and rapid turbidimetric method for the assay of hyaluronidase (1) has proved of great aid in the purification of this enzyme from bovine testes (2). Chondroitinsulfuric acid (CSA) is related chemically and biologically to hyaluronic acid, the substrate of hyaluronidase. The presence of a previously identified (3) CSA-depolymerizing activity (chondroitinase) in crude testicular preparat...